Evaluation of the protective effect of coded plant leaf ethanolic extract (222) against paracetamol-induced hepatotoxicity and oxidative stress in Wistar albino rats - Part III

Abstract

In this part, the protective effect of the ethanolic leaf extract of coded plant (Code No. 222*) was evaluated against paracetamol (APAP)-induced hepatotoxicity and oxidative stress in Wistar rats. In toxin control paracetamol treated group, elevated levels of serum enzymes are indicative of cellular leakage and loss of functional integrity of cell membrane in the liver. But the elevated levels of the serum enzymes are decreased to near normal levels in the 222 extract treated groups. The alanine aminotransferase (ALT), aspartate aminotransferase (AST) and triglyceride levels are significantly (P≤0.05) decreased in 222 (125 mg/kg) to normal level comparable to the standard control, silymarin. The levels of cholesterol and triglyceride were significantly (P≤0.05) increased in paracetamol treated rats. Administration of 222 at 125 mg/kg reduces the cholesterol and triglyceride levels significantly (P≤0.05), comparable to the standard control silymarin. Further, there was a significant (P≤0.05) increase in the hepatic antioxidant enzyme status of 222 extract (125 mg/kg) treated animals compared to standard control group against the oxidative stress generated by paracetamol. Histopathological studies of the liver samples confirmed the hepatoprotective property of the coded drug 222. It was also seen that histopathological damage induced by paracetamol were improved in rat liver, treated with the extract. The results of the present study revealed the hepatoprotective potential of coded plant (222) leaf extract against paracetamol induced hepatotoxicity and oxidative stress in Wistar rats.